Safe | Accurate | Easy on the Patient



  • Blood based, Non-invasive
  • Safe
  • Accurate
  • Easy on the Patient
  • Reasonable Charges
  • Available for all solid organs


Symptomatic individuals who have been advised a biopsy to check for malignancy.
Patients where an invasive biopsy has been inconclusive or inconsistent with clinical observations.
Suspected metastatic relapse to rule out new primary.


The anxiety, pain, risks and costs associated with invasive biopsies for cancer diagnosis are substantial. A reliable, safe and convenient test to establish diagnosis in suspected cases of cancer has not been available.

Trublood® has been clinically validated on more than 40,000 samples from patients and healthy individuals to whom we are ever grateful. Trublood® is a revolutionary non-invasive, safe and accurate blood test that can substitute invasive biopsies in most suspected cases of solid organ cancers. With a simple 20-25 ml of blood draw, the Trublood® analysis involves extremely sensitive, sophisticated and careful isolation and analysis of ‘live’ tumor cells and Nucleic Acid Fragments (DNA / RNA) from the patient’s blood.

A comprehensive report is provided with unprecedented level of information which was hitherto thought impossible. Trublood® can be repeated as often as necessary, even during treatment or thereafter for real time characterization and personalization of treatment.

Trublood® is a result of several years of research put in by more than 150 of our scientists and clinicians working as a team using the world’s latest equipment and software.

Trublood® is a new paradigm in cancer diagnosis and management.


What Non-Invasive Diagnostic biopsy to substitute invasive tissue extraction.
For Whom Symptomatic individuals who have been advised a biopsy to check for malignancy. Patients where an invasive biopsy has been inconclusive or inconsistent with clinical observations. Suspected metastatic relapse to rule out new primary.
Why Invasive biopsies are risky, inconvenient, painful and must be performed in a clinical setting. Trublood sample can be collected from patient’s house or office.
How Circulating Tumor Cells and Nucleic Acid are isolated from patient’s blood samples and extensively analysed for diagnosis, prognosis and theranostics.
Sample Type 17 / 25 ml peripheral blood (5-6 hours fasting) as per protocol depending upon extent of test.
Turn Around Time 5 days


Usual Tissue Biopsy / FNAC Trublood
Invasive, needs tissue and is ultimately expensive Totally non invasive and is ultimately less expensive
Can be performed only in Hospital / with Anesthesia No need for Hospitalization / Anesthesia
Usually painful, may need stitches and leave scars No Pain, No Stitches, No Scars
Serious risk of tumor cell ‘Seeding’ No Risk
Can be very risky for organs like Lung, Liver, Pancreas No risk of injury to any organ / bleeding
May be misleading as it is site / time dependent Provides ‘Real time’ data and covers all active sites
Serial / sequential biopsies are impossible Can be performed as often as necessary
Not viable if primary tumor is not easily visualized Viable even if primary / metastasis are undetectable



Cancer Type Analysis Type
Head and Neck

* Optional at extra cost.



1st Draw
2ml SST Tube
(Yellow colour cap).

2nd Draw
3 x EDTA Tubes (Purple Colour Cap)
of 5 ml each – total 15 ml.

Thus, total 4 tubes containing 17 ml whole blood.


1st Draw
2ml SST Tube
(Yellow colour cap).

2nd Draw
8ml DCG Tube
(Brown Colour Cap).

3rd Draw
3 x EDTA Tubes (Purple Colour Cap)
of 5 ml each – total 15 ml.

Thus, total 5 tubes containing 25 ml whole blood.

Note :

    • Sequence of draw should not be altered.
    • Blood should be drawn only and only as per above method.
    • Blood drawn should be performed only by qualified phlebotomist under medical supervision.
    • Ship 4 °C in the container provided by DCG.


  • The patient must not have received any form of cancer therapy (radiation / chemotherapy / surgery / endocrine etc.) at least 30 days prior to collection of sample.
  • The patient must not have received oral or IV corticosteroids at least 14 days prior to collection of sample.
  • Patient has no current febrile or any other acute inflammatory illness.
  • Patient does not have acute exacerbation or flareup of an inflammatory condition requiring escalation in medical therapy at least 5 days prior to collection of sample.
  • Patient has not received blood transfusion / PET-CT / CTscan at least 5 days prior to collection of sample.
  • Patient is not positive for HIV / HBV / HCV.


  • PD-L1, Cell Free DNA + RNA, FISH and Pharmacogenetics analysis will be performed on a special request at extra cost.
  • Turn Around Time (TAT) for above report is 10 working days.


Trublood® non-invasive diagnostic biopsy for solid organ cancers has been developed by Datar Cancer Genetics based on the findings of two clinical trials registered with the CTRI (Registration No. CTRI/2019/01/017219 and CTRI/2019/03/ 017918).

Trublood has been extensively validated with data from more than 22,000 samples from asymptomatic individual donors who underwent currently used screening tests such as LDCT, Mammography, PAP Smear, Serum CA Markers and clinical examinations, as well as more than 18,000 samples from cancer patients / patients with benign conditions totalling more than 40,000 evaluable samples till December, 2019.


Cancer Type Patients
Breast 3967
Head and Neck 3552
Lung 1378
Colorectal 1341
Prostate 1196
Cervix 930
Ovary 855
Esophagus 507
Sarcoma 440
Stomach 392
Uterus + Endometrium 365
Pancreas 385
Liver 381
CNS 349
Kidney 342
Bladder 249
Bone 220
Gallbladder 196
Thyroid 175
Testes 135
Skin 115
Melanoma 86
Penis 72
Neuroendocrine 51
Others 154
Total 17,833


Particulars Samples
All Cancers 17,833
Benign Conditions 488
Asymptomatic Individuals 22,030
Total 40,351


  • Tumors release thousands of cells into the circulation, where circulating tumor cells (CTCs) survive for about 1–2.5 hours.
  • In order to detach from the primary tumor and disseminate into the blood, cells must undergo a cellular process known as Epithelial-Mesenchymal Transition (EMT).
  • EMT enhances migratory capabilities of tumor cells, which allows cells to penetrate into the vasculature and circulate as single or clusters of circulating tumor cells (CTCs).
  • CTCs extravasate having undergone the reverse process known as Mesenchymal to Epithelial Transition (MET) and colonize at distant organs.
  • Circulating Tumor Cells (CTCs) are defined as EpCAM (+), PanCK (+), CD45 (-) cells. Circulating Tumor Associated Cells (C-TACs) are EpCAM(+), PanCK(+), CD45(+/-) cells of tumorigenic origin, in peripheral blood.
  • Non-tumorigenic cells in peripheral blood have functional apoptotic mechanism, but CTC and C-TACs are resistant to apoptosis.
  • An epigenetically active stabilizing process can eliminate normal cells and confer survival privilege on apoptosis – resistant C-TACs, CTC and C-TACs.
  • Sufficient C-TACs can be enriched and harvested for Immunocytochemistry (ICC) profiling with markers used in immunohistochemistry (IHC) which aid in determination of histopathological subtypes of tumor tissue.
  • Antibody clones used in the trublood® assay for analysis of tumor antigens/markers are internationally approved for IVD use.


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Terms and Conditions

On my request, Datar Cancer Genetics (DCG) has agreed to permit me to access and download a copy of the specimen Test Report for 'Trublood' on the following express conditions which I hereby declare are binding on me and also declare that but for such acceptance by me, DCG would not have permitted me to download and obtain printout of the said specimen report. I have read the Terms and Conditions set out below carefully and after full consideration and understanding my responsibility, and the fact that the information is provided to me for my bona fide personal use and made available in 'trust', I subscribe to the same as under:

Terms and Conditions:

  • I have correctly declared my true legal name in the information submitted at the time of my request.
  • That I am a person above the age of 18 years and legally competent to enter into a lawful and binding contract.
  • That I expressly recognize and do not dispute that DCG is the sole Copyright holder and Intellectual Property holder in respect of all contents in regard to the test procedure, the process of analysis, layout and contents of the Report, the design and artwork and all such other intangible and intellectual property of DCG which is materially related to the said Report and I undertake not to infringe and/or prejudicially use and/or disclose to any third person the said information / report / material particulars to the prejudice of DCG.
  • That I shall not directly or indirectly derive any commercial benefit from the said material so downloaded by me and/or any intellectual property related thereto.
  • I recognize and declare that in case of breach of any of the aforesaid express covenants and all the implied duties of a trustee, DCG would be entitled to both monetary and injunctive reliefs against me and I consent to passing an injunctive order against me in the event of infringement / breach of the aforesaid undertakings and declarations.
  • I solemnly declare as above.


Can Trublood® detect all cancers?

Trublood® detects presence of carcinoma i.e. epithelial malignancies, melanoma as well as certain subtypes of sarcoma. It does not detect haematolymphoid malignancies. Carcinomas are the most common malignancy in adults.

Can Trublood® differentiate between squamous cell carcinoma and adenocarcinoma?

Can Trublood® always provide specific histopathological subtype? Can it identify rare HPE subtype?

Can Trublood® give us the histological grade of the tumor?

What is the principle used in Trublood®?

How is Trublood® different from a Circulating Tumor Cell (CTC) test?

Can I use Trublood® for longitudinal CTC enumeration?

Can you find CTCs in blood when there is Carcinoma in situ? / Can Trublood® identify carcinoma in situ?

Can you find CTCs in early stage cancer?

How does it give guidance for therapy?

How does it differ from other cancer screening methods e.g. CA125, CA19-9, etc.?

Can Trublood® be offered to a person with non-specific cancer symptoms such as cachexia without clinical suspicion of a particular organ involvement?

How is the sample collected? Is there any special procedure for collection?

How is the sample transported?

What is the next step if Trublood® gives positive result?

Do I need to confirm the presence of malignancy with an invasive biopsy when Trublood® results are positive?

Do you have any comparative data?

How is the performance of Trublood®? What is the validation you have for this test?

Is it being used by clinicians anywhere?

What if the test is inconclusive?

Can Trublood® be availed for childhood malignancies?

What advantage does Trublood® offer over tissue biopsy?

What are the limitations of Trublood®?

Can Trublood® give theragnostic information similar to that obtained by tissue biopsy?

Will doctor treat me based on Trublood® result?

Can Trublood® be used in haematological malignancies?


I have read and understood the terms of use and the privacy policy and I agree.